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以常规的酶联免疫吸附法为基础,不断改进,形成了多种酶联免疫吸附法,如模块化ELISA,双位点一步法,捕获法测IgM抗体,生物素、亲和素系统,斑点酶链免疫吸附试验,硝酸纤维素膜微粒ELISA等方法。酶链免疫吸附法在医药,食品加工业,农牧渔业等领域有着广泛的应用:如乙型肝炎,莱姆病,急性心肌梗死的早期诊断,定量检测内毒素,HIV抗体初筛,SARS病毒的快速检测;检测食品中的毒素,残留农药,微生物及其它成分;检测香蕉有关病毒,小麦黄花叶病毒,棉花黄萎病菌毒素,转Bt基因抗 虫棉,检测水产品中氯霉素的残留量,猪细小病毒(PPV)血清抗体,禽脑脊髓抗体等。
Abstract：Routine ELISA as the foundation,update and form various ELISA,such as blocking ELISA ,both bit one step ,Mac ELISA examine IgM, Biotin Avidin system, Dot ELISA, nitrocellulose microparticle ELISA, etc.ELISA technique is extensively used in medicine, foodstuff machining, agriculture and fishery field,etc., such as the early diagnosis of HBV, Lyme disease, acute myocardium infarct, ration measuring of endotoxin, HIV antibody sift for the first time, fast measuring of SARS virus; the toxin measuring in the food, the remains of pesticide, microorganism and other composition; measuring the relevant virus of banana, wheat yellow mosaic virus, verticillium dahliae phytotoxin of cotton, transferred Bt gene resisting the worm cotton, measureing the residue amount of the chloramphenicol of the aquatic products, serum antibody of porcine parvovirus(PPV), avian encephalomyelitis(AE) antibody,etc.
[1]张莹,徐文弟.酶联免疫吸附法在医学研究中的新进展[J].哈尔滨医科大学学报,2000,(8):306-308.
[2]BraneKrt.Development and evaluation of variousClostridium perfringensβ anti-toxins[J].FEMS Immunology andMedicalMicrobiology,24,(1999):293-297.
[3]佚名.酶联免疫技术基础知识[DB/OL].上海贝西科技发展有限公司·上海贝西[DB/OL]. http://www.beixi.com/cn/support.htm.200107-08/2003-04-12.
[4]刘国霞,曾宪垠.酶联免疫吸附测定技术新进展[J].河南畜牧兽医,2001,(7):13-14.
[5]邹静.硝酸纤维素膜微粒ELISA方法的建立[J].免疫学杂志,1999,(2):143.
[6]李振林.微生物学及检验技术(第二版)[M].广州:广东科技出版社,1994:119-120.
[7]贾月萍.ELISA检测莱姆病特异性抗体IgG[J].中国微生物学和免疫学杂志,2000,(1):118.
[8]刘宏伟,仲伟强,王俊朝.血清肌红蛋白快速酶免疫分析诊断急性心肌梗死[J].免疫技术与临床,2001,(6):92-95.
[9]石怡珍,施辛,杨辰ELISA检测梅毒IgM抗体在早期梅毒诊断中的应用[J],免疫技术与临床,2001,(10):202-204.
[10]SchrnidtB L.EdilalipourM.LugerA.Comparative evaluation of nine different enzyme-linked immunosorbent assays for determination of antibodies treponema pallidum in patients with primauy syphilis[J].2000J ClinMicrobiol,2000,38(3):1279-1282
[11]RitaCastro,Em韑iaS.Prieto,Evaluation of anEnzymeImmunoassayTechnique forDetection ofAntibodies againstTreponema pallidum[J],journal of clinicl microbiologyJan.2003:250-253.
[12]王义清.应用双抗原夹心ELISA法筛查献血员梅毒螺旋体抗体[J].放射免疫血杂志,2002,(5):318-319.
[13]徐修礼,薛采芳,于文彬.双抗体夹心ELISA定量检测内毒素的方法学研究[J].细胞与分子免疫血杂志,2002,(2):175-177.
[14]佚名.HIV抗体初筛实验[DB/OL].艾滋病在线·诊断检测[DB\OL].http://www.aidsonline.com.cn/check/check_01.htm.2002-08-12/2003-04-09.
[15]佚名.艾滋病及其试验诊断进展[DB/OL].检验天空·学术交流·免[DB/OL].http://www.labsky.com/data/02/12 /communion/1223133400978.htm.2003-12-23/2003-03-02.
[16]陈平.非典真面目这样揭开[N].楚天都市报,2003-04-24-(8).
[17]朱慧莉,黎锡流,许喜林酶联免疫吸附法及其在食品分析中的应用[J].食品工业科技,2001,(2):80-82.
[18]周培,陆贻通.农药残留的酶联免疫检测技术研究进展[J].环境污染与防治,2002,(8):248-251.
[19]佚名.组培苗生产中必须注意的几个问题[DB/OL].中国水果·香蕉网·香蕉种植管理[DB/OL].http://www. efruit.com.cn/xjw/mfy/zzg149.htm./2003-04-25.
[20]尚巧霞,韩成贵,杨莉莉.小麦黄花叶病毒的血清学检测[J].北京农学院学报,2002,(4):15-17.
[21]刘凤权,章元寿.棉花黄萎病菌毒素ELISA检测方法的建立及其应用[J].棉花学报,2003,(1):47-50.
[22]陈松,张震林,张香桂,等.ELISA方法鉴定转Bt基因抗虫棉[J].江苏农业科学,2003,(1):21-23.
[23]沈美芳,赵文亚,费志良,等.用酶联免疫法测定水产品中氯霉素的残留量[J].南京师范大学学报(工程技术版),2003,(2):1-4.
[24]付利芝,王红宁,曹国文,等.间接Dot-PPA-ELISA检测猪细小病毒血清抗体[J].中国兽医杂志,2003,(6):12-15.
[25]秦卓明,赵继勋,杨建民,等.酶联免疫吸附试验检测禽脑脊髓炎抗体方法的研究[J].山东农业科学,2002,(1):13-16.
基本信息:
中图分类号:R392-33
引用信息:
[1]李文敏.酶联免疫吸附反应的技术进展及应用[J].湖北职业技术学院学报,2003(04):65-69.